LOCUS       40924_18801        3602 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pExchange 1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Carstens C-P., Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2000) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 3602)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3602)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (12-DEC-2000) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    651..758
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(780..798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1072..1193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1200..1655)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1682..1784
                     /label=AmpR promoter
     protein_bind    1801..1834
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1879..2736)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2906..3494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc
      721 ttatcgatac cgtcgacctc gagggggggc ccggtaccag gtaagtgtac ccaattcgcc
      781 ctatagtgag tcgtattaca attcactcga tcgcccttcc caacagttgc gcagcctgaa
      841 tggcgaatgg agatccaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
      901 tgtgtatttt agattcacag tcccaaggct catttcaggc ccctcagtcc tcacagtctg
      961 ttcatgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     1021 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     1081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     1201 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     1261 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     1321 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     1381 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     1441 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     1501 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     1561 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     1621 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     1681 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1741 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagaatcctg aggccgggcc
     1801 ataacttcgt ataatgtatg ctatacgaag ttatccatgg gccccccctc gacatgagta
     1861 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1921 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1981 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2041 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2101 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2161 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2221 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2281 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2341 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2401 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2461 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2521 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2581 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2641 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2701 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctcaggt tactcatata
     2761 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2881 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2941 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3001 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3061 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3601 cc
//
